abstract：:A reliable method for high-resolution HLA-DQB1 typing using a combination of PCR- restriction fragment length polymorphism (RFLP) and PCR-single strand conformation polymorphism (SSCP) analysis is described. The second exon of the DQB1 gene was subjected to PCR using generic primers and digested with two restriction e...

journal_title：Human immunology

authors： Park MH,Whang DH,Kang SJ

更新日期：1999-09-01 00:00:00

abstract：:There are clinical situations in which it may be advantageous to monitor delayed type hypersensitivity (DTH) responses, an index of cell-mediated immunity, without exposing patients directly to the challenge antigens. For example, transplant patients may be at risk for becoming sensitized to donor antigens if injected...

journal_title：Human immunology

authors： Carrodeguas L,Orosz CG,Waldman WJ,Sedmak DD,Adams PW,VanBuskirk AM

更新日期：1999-08-01 00:00:00

abstract：:The expression of the Duffy Antigen/Receptor for Chemokines (DARC) on red blood cells (RBC) has been commonly determined using hemagglutination tests. Because the vast majority of African individuals are Duffy-negative, screening for DARC expression on RBC is a valuable tool to assess Caucasian admixture in population...

journal_title：Human immunology

authors： Nickel RG,Willadsen SA,Freidhoff LR,Huang SK,Caraballo L,Naidu RP,Levett P,Blumenthal M,Banks-Schlegel S,Bleecker E,Beaty T,Ober C,Barnes KC

更新日期：1999-08-01 00:00:00

abstract：:The HER-2/neu protein is overexpressed in approximately 20% of human adenocarcinomas and is a defined tumor antigen in breast cancer. The purpose of this study was to evaluate the endogenous HER-2/neu specific antibody response in 57 patients with colorectal cancer. HER-2/neu specific antibodies, titer > or = 1:100, w...

journal_title：Human immunology

authors： Ward RL,Hawkins NJ,Coomber D,Disis ML

更新日期：1999-06-01 00:00:00

abstract：:Heart transplant rejection is routinely defined by histological evaluation of endomyocardial biopsies (EMB). As elevated levels of donor derived sHLA (dsHLA) can be detected in the serum of transplanted patients just before or during rejection, quantification of donor specific soluble counterparts of HLA Class I (sHLA...

journal_title：Human immunology

authors： Koelman CA,Ensink W,Mulder A,Tanke J,Doxiadis II,Claas FH

更新日期：1999-05-01 00:00:00

abstract：:The cellular basis of graft rejection and the development of strategies for specific suppression of T cell responses against allogeneic and xenogeneic transplants represents an area of active investigation. Recently, a population of MHC-class I restricted CD8+CD28- T suppressor cells (Ts) which are able to inhibit spe...

journal_title：Human immunology

authors： Pennesi G,Liu Z,Ciubotariu R,Jiang S,Colovai A,Cortesini R,Suciu-Foca N,Harris P

更新日期：1999-04-01 00:00:00

abstract：:There is a growing body of information about the soluble forms of HLA in serum but there are only a few reports discussing sHLA in other body fluids. We quantitated sHLA-I and sHLA-II concentrations in sweat, saliva and tear samples from five normal individuals with known HLA-phenotypes. We also studied sweat samples ...

journal_title：Human immunology

authors： Aultman D,Adamashvili I,Yaturu K,Langford M,Gelder F,Gautreaux M,Ghali GE,McDonald J

更新日期：1999-03-01 00:00:00

abstract：:We have characterized the T lymphocyte population of the human neonate in respect of the expression of phenotypic profiles for naive, memory and differentiated populations. We have examined the response of the neonate T cell to the superantigen Staphylococcus enterotoxin B (SEB) and compared the response to T cells fr...

journal_title：Human immunology

authors： Macardle PJ,Wheatland L,Zola H

更新日期：1999-02-01 00:00:00

abstract：:We describe a two-step clonal culture assay system for human lymphohematopoietic progenitors present in umbilical cord blood which are capable of differentiation along both myeloid and B-lymphoid lineages. Human cord blood CD34+ cells were plated in methylcellulose in the presence of stem cell factor (SCF), granulocyt...

journal_title：Human immunology

authors： Yoshikawa Y,Ikebuchi K,Ohkawara J,Hirayama F,Yamaguchi M,Sato N,Mori KJ,Kasai M,Sekiguchi S

更新日期：1999-01-01 00:00:00

abstract：:The nonclassical MHC class I HLA-G antigen is expressed in cytotrophoblasts during pregnancy and may play a role in inhibiting lysis by maternal natural killer cells. HLA-G gene transcription was analyzed in human fetal liver of 6-8 wk of gestation, a development stage where classical HLA class I expression is very re...

journal_title：Human immunology

authors： Moreau P,Lefebvre S,Gourand L,Dausset J,Carosella ED,Paul P

更新日期：1998-12-01 00:00:00

abstract：:Interactions between human IgG with human FcgammaRI and FcgammaRIIa (R131) were studied to investigate the role of the hinge region of IgG3 and IgG1 in the binding of the antibodies to FcgammaR. It was found that a hinge deletion mutant of IgG3 (IgG3 m15) was reduced in its ability to bind to FcgammaRI and FcgammaRIIa...

journal_title：Human immunology

authors： Redpath S,Michaelsen TE,Sandlie I,Clark MR

更新日期：1998-11-01 00:00:00

abstract：:The presentation of donor-derived MHC peptides by recipient APCs to T cells is an essential component of the rejection of allografts (indirect allorecognition). Initial alloreactive T cell response is confined to a few well processed and presented dominant determinants on donor MHC. However, during long-term graft rej...

journal_title：Human immunology

authors： Benichou G,Malloy KM,Tam RC,Heeger PS,Fedoseyeva EV

更新日期：1998-09-01 00:00:00

abstract：:To elucidate the residues important for the binding of peptides to HLA-Cw3, a substitutional analysis of two HLA-Cw*0304-binding peptides was performed. The optimal registry and length for a Cw3-restricted epitope from HIV-1 p24gag was determined to be a nonamer, p24gag 144-152. Substituted analogs of this nonamer pep...

journal_title：Human immunology

authors： Zarling AL,Lee DR

更新日期：1998-08-01 00:00:00

abstract：:Rat soluble MHC class I synthesis was studied at both RNA and protein levels to determine whether multiple forms of soluble MHC class I molecules are produced by different mechanisms. RT-PCR and sequencing of MHC class I transcripts identified an alternatively spliced nonclassical MHC class I gene product, lacking bot...

journal_title：Human immunology

authors： Zhai Y,Knechtle S

更新日期：1998-07-01 00:00:00

abstract：:Recurrent Spontaneous Abortion (RSA) is postulated to be due to several factors including immunogenetic mechanisms. Many studies have been conducted on the effect of the MHC region in the reproductive phenomena suggesting an immunological or genetic involvement in RSA. We studied couples with 3 or more abortions among...

journal_title：Human immunology

authors： Pennesi G,Brioli G,Lulli P,Mariani B,Morellini M,Nicotra M,Trabace S

更新日期：1998-06-01 00:00:00

abstract：:A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens ar...

journal_title：Human immunology

authors： Pei R,Wang G,Tarsitani C,Rojo S,Chen T,Takemura S,Liu A,Lee J

更新日期：1998-05-01 00:00:00

abstract：:HLA exhibits the most extensive polymorphism of any of the known human genes and is known as a genetic marker which allows genetic background of many diseases and physical phenomena. In this study, we, therefore, tried to investigate the regulation of HLA polymorphism and peak bone mass (PBM) in order to elucidate the...

journal_title：Human immunology

authors： Tsuji S,Munkhbat B,Hagihara M,Tsuritani I,Abe H,Tsuji K

更新日期：1998-04-01 00:00:00

abstract：:Peptide binding to HLA-DR molecules in intracellular compartments is facilitated by HLA-DM molecules, present in most types of antigen-presenting cells. Allorecognition of DR specificities represents a form of T cell recognition of the MHC-peptide complex which in some cases is influenced by peptide binding. DRA and D...

journal_title：Human immunology

authors： Drover S,Kovats S,Masewicz S,Blum JS,Nepom GT

更新日期：1998-02-01 00:00:00

abstract：:Conventional matching is based on numbers of alleles shared between donor and recipient. This approach, however, ignores the degree of relationship between alleles and haplotypes, and therefore the actual degree of difference. To address this problem, we have compared family members using a block matching technique wh...

journal_title：Human immunology

authors： Bellgard MI,Tay GK,Hiew HL,Witt CS,Ketheesan N,Christiansen FT,Dawkins RL

更新日期：1998-01-01 00:00:00

abstract：:A PCR-based SSO-assay has been developed to characterize the allelic polymorphism at the HLA-DPA1 locus. To validate the performance of this assay, 77 samples were typed side by side in a blinded fashion by the SSO assay and sequencing-based typing (SBT); 100% concordance was seen between the two methods. To address q...

journal_title：Human immunology

authors： Moonsamy PV,Klitz W,Tilanus MG,Begovich AB

更新日期：1997-12-01 00:00:00

abstract：:We compared the T cell receptor (TCR) V beta gene family repertoire in peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells from 7 human immunodeficiency virus (HIV)-infected patients and 3 seronegative healthy controls. Virtually all the V beta family specificities were represented in patient PBMC and ...

journal_title：Human immunology

authors： Mion M,Indraccolo S,Feroli F,Minuzzo S,Masiero S,Zamarchi R,Barelli A,Borri A,Chieco-Bianchi L,Amadori A

更新日期：1997-10-01 00:00:00

abstract：:The HLA-A, HLA-B and HLA-C molecules have turned out to be highly polymorphic and functionally complex. They not only serve as peptide receptors, but also interact with beta 2-microglobulin, an alpha beta T cell receptor, CD8 and NK inhibitory molecules, all at different sites. The fact that more than 300 class I alle...

journal_title：Human immunology

authors： Kostyu DD,Hannick LI,Traweek JL,Ghanayem M,Heilpern D,Dawson DV

更新日期：1997-09-15 00:00:00

abstract：:Immunoglobulin idiotypes (Id) of malignant B cells represent highly specific markers which can be used for vaccination. PCR-amplification of immunoglobulin genes enables the rapid production of large amounts of Id vaccines. However, the separate amplification and subsequent recombination of heavy and light chains can ...

journal_title：Human immunology

authors： Terness P,Welschof M,Moldenhauer G,Jung M,Moroder L,Kirchhoff F,Kipriyanov S,Little M,Opelz G

更新日期：1997-08-01 00:00:00

abstract：:A murine monoclonal antibody (mAb), 928, that recognizes a cell surface antigen (928 Ag) on a human Epstein-Barr virus-transformed fetal liver-derived lymphoid progenitor cell line (FL4.4) was generated. The 928 mAb reacted with only FL4.4; it did not react with any other 57 cell lines tested. Two color flowcytometry ...

journal_title：Human immunology

authors： Kishi H,Okumura A,Tong JJ,Sugiyama E,Matsuno H,Minowada J,Kanai T,Nishimura Y,Muraguchi A

更新日期：1997-08-01 00:00:00

abstract：:Two members of a unique class of natural antibodies have been identified in all of a large cohort of sera from clinically normal humans of broad age distribution. By means of a series of 10-12 mer peptides the epitope for each of those antibodies was characterized with regard to amino acid identity and conformation. S...

journal_title：Human immunology

authors： Rodman TC,To SE,Sullivan JJ,Winston R

更新日期：1997-07-01 00:00:00

abstract：:Our objective was to study a possible contribution of MHC genes to S-HLA-I secretion in patients with Type I diabetes. Quantitatively, we used a highly sensitive enzyme-linked immunoassay to measure S-HLA-I in the serum of a total of 39 patients with Type I diabetes, as well as 36 kinships of 12 diabetic patients and ...

journal_title：Human immunology

authors： Adamashvili I,McVie R,Gelder F,Gautreaux M,Jaramillo J,Roggero T,McDonald J

更新日期：1997-07-01 00:00:00

abstract：:Population studies represent an integral part, and a necessary link in a complex chain of host-pathogen interactions, disease pathogenesis, and major histocompatibility complex polymorphism. HLA class I and class II allele and haplotype distributions among Venezuelan mestizos were determined. Genes of Mongoloid, Negro...

journal_title：Human immunology

authors： Makhatadze NJ,Franco MT,Layrisse Z

更新日期：1997-06-01 00:00:00

abstract：:Antigen-selected T cell receptor (TCR) repertoires vary in complexity from very limited to extremely diverse. We have previously characterized two different CD8 T cell responses, which are restricted by the same mouse major histocompatibility complex (MHC) class I molecule, H-2 Kd. The TCR repertoire in the response a...

journal_title：Human immunology

authors： Maryanski JL,Casanova JL,Falk K,Gournier H,Jaulin C,Kourilsky P,Lemonnier FA,Lüthy R,Rammensee HG,Rötzschke O,Servis C,López JA

更新日期：1997-05-01 00:00:00

abstract：:An HLA-E polymorphism study by oligotyping and DNA sequencing was carried out in the Spanish population. As a result, a new HLA-E allele (E*01031) initially assigned by polymerase chain reaction oligotyping as E*0104 was found. This allele presents a synonymous change at codon 77 (AAT-->AAC; Asn) when compared with th...

journal_title：Human immunology

authors： Gomez-Casado E,Martinez-Laso J,Vargas-Alarcón G,Varela P,Diaz-Campos N,Alvarez M,Alegre R,Arnaiz-Villena A

更新日期：1997-04-15 00:00:00

abstract：:Class I molecules of the major histocompatibility complex bind peptides derived from cytosolic proteins and display them on the cell surface. This function alerts cytotoxic T cells to the presence of intracellular pathogens. Class I molecule assembly requires the association of the heavy chain with beta 2-microglobuli...

journal_title：Human immunology

authors： Bresnahan PA,Barber LD,Brodsky FM

更新日期：1997-04-01 00:00:00

abstract：:M proteins are coiled-coil dimers expressed on group A streptococcal cell surfaces. They have an important role in host antistreptococcal immunity and in poststreptococcal autoimmune sequelae. Controversy has arisen regarding whether type 5 M proteins are superantigenic for human T cells. To investigate this, we have ...

journal_title：Human immunology

authors： Degnan B,Taylor J,Hawkes C,O'Shea U,Smith J,Robinson JH,Kehoe MA,Boylston A,Goodacre JA

更新日期：1997-04-01 00:00:00

abstract：:T-cell clonal anergy induced by peptide analogs in the presence of live APC in murine systems was reported to be associated with incomplete tyrosine phosphorylation of the CD3 zeta chain followed by a lack of subsequent ZAP-70 recruitment. Furthermore, protein tyrosine phosphatase SHP-1 was associated with ZAP-70 upon...

journal_title：Human immunology

authors： Matsushita S,Nishimura Y

更新日期：1997-03-01 00:00:00

abstract：:The clonal selection theory of immune reactivity is based on a metaphor of self/not-self discrimination and considers self-tolerance to result from clonal deletion. There is evidence that a deletional mechanism is responsible for negative selection of self MHC-reactive T-cells in the thymus. Bretscher/Cohn theory buil...

journal_title：Human immunology

authors： Lafferty KJ,Gazda LS

更新日期：1997-02-01 00:00:00

abstract：:To calculate reliable HLA gene and haplotype frequencies of bone marrow donors in various regions in the world, we have analyzed the HLA-A, -B, and -DR phenotype frequencies of 18 bone marrow donor registries with a total of more than 300,000 HLA-A, -B-typed donors. These registries were included in the 22nd edition o...

journal_title：Human immunology

authors： Schipper RF,D'Amaro J,Bakker JT,Bakker J,van Rood JJ,Oudshoorn M

更新日期：1997-01-01 00:00:00

abstract：:The difficulty of molecular typing of the HLA class I genes and the relevance of the genes of this region to disease susceptibility and transplantation have provided an impetus to develop useful typing markers. We have characterized by polymerase chain reaction analysis a new highly informative CA repeat localized app...

journal_title：Human immunology

authors： Grimaldi MC,Clayton J,Pontarotti P,Cambon-Thomsen A,Crouau-Roy B

更新日期：1996-12-01 00:00:00

abstract：:Prevention of rejection and the induction of transplantation tolerance are two related but separable phenomena that must both be considered in the analysis of the response to a transplanted organ. It is frequently hard to separate these phenomena in assessing the outcome of clinical transplants, because patients are r...

journal_title：Human immunology

authors： Gianello PR,Sachs DH

更新日期：1996-09-15 00:00:00

abstract：:The capacity of MHC class I to protect target cells from NK is well established, but the mechanism by which these molecules influence NK recognition and the physical properties associated with this function remain poorly defined. We have examined this issue using as a model the HLA-A11 allele. HLA-A11 expression corre...

journal_title：Human immunology

authors： Gavioli R,Zhang QJ,Masucci MG

更新日期：1996-08-01 00:00:00

abstract：:To identify the existence of antigen specific T cell responses and to follow the changes of these reactions, it is considered useful to evaluate whether certain T cells clonally accumulate in the lymphocyte population. For this purpose, we have established a novel method to analyze T cell clonality using a combination...

journal_title：Human immunology

authors： Yamamoto K,Masuko-Hongo K,Tanaka A,Kurokawa M,Hoeger T,Nishioka K,Kato T

更新日期：1996-06-01 00:00:00

abstract：:To identify disease-specific T cell changes that occur in Crohn's disease (CD) the T-cell receptor (TCR) BV repertoires of lamina propria lymphocytes (LPL) from both disease-active and disease-inactive colonic tissue of three CD patients were compared by a quantitative polymerase chain reaction (qPCR) and CDR3 length ...

journal_title：Human immunology

authors： Gulwani-Akolkar B,Akolkar PN,Minassian A,McKinley M,Fisher S,Silver J

更新日期：1996-06-01 00:00:00

abstract：:Platelet alloimmunization may result in post-transfusion purpura, and during pregnancy may cause neonatal alloimmune thrombocytopenia (NAIT), with a frequency estimated at 1.3 per 1000 live births. The risk of morbidity is significant: 20% of affected infants have neurologic sequelae and the death rate is about 10%. A...

journal_title：Human immunology

authors： Semana G,Zazoun T,Alizadeh M,Morel-Kopp MC,Genetet B,Kaplan C

更新日期：1996-04-01 00:00:00